I've gottan urine on tests that I have taken and it never effected ne thing, so it probably didnt. If ur worried that it did go buy another one and this time pee in a cup or something ur not gonna reuse and stick the test thing in it and then see what the results are. If you dont want to spend the money on another one just go to a clinic and see if you can take a pregnancy test. After I took my first test and it was positive I went to birthrite and took another test to make sure and that was free, unlike those darn tests.